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In-line buffer exchange in the coupling of Protein A chromatography with weak cation exchange chromatography for the determination of charge variants of immunoglobulin G derived from chinese hamster ovary cell cultures

https://doi.org/10.1016/j.chroma.2024.464722

Wysor, Sarah K; Synoground, Benjamin F; Harcum, Sarah W; Marcus, R Kenneth (March 2024, Journal of Chromatography A)

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Method for high-efficiency fed-batch cultures of recombinant Escherichia coli

https://doi.org/10.1016/bs.mie.2021.05.004

Caldwell, Thomas P.; Synoground, Benjamin F.; Harcum, Sarah W. (October 2021, Methods in enzymology)

Fed-batch processes are commonly used in industry to obtain sufficient biomass and associated recombinant protein or plasmids. In research laboratories, it is more common to use batch cultures, as the setup of fed-batch processes can be challenging. This method outlines a robust and reliable means to generate Escherichia coli biomass in a minimum amount of fermentation time using a standardized fed-batch process. Final cell densities can reach over 50g dry cell weight per liter (g dcw/L) depending on the strain. This method uses a predefined exponential feeding strategy and conservative induction protocol to achieve these targets without multiple trial and error studies. If desired, productivity can be optimized by balancing the induction time and feed rates. This method utilizes cost-efficient defined media, minimizes process control complexity, and potentially aids downstream purification.
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Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles

https://doi.org/10.1002/biot.202100098

Synoground, Benjamin F.; McGraw, Claire E.; Elliott, Kathryn S.; Leuze, Christina; Roth, Jada R.; Harcum, Sarah W.; Sandoval, Nicholas R. (July 2021, Biotechnology Journal)

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Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturing

https://doi.org/10.1002/bit.28290

Cordova, Lauren T.; Dahodwala, Hussain; Elliott, Kathryn S.; Baik, Jongyoun; Odenewelder, Daniel C.; Nmagu, Douglas; Skelton, Bradley A.; Uy, Lisa; Klaubert, Stephanie R.; Synoground, Benjamin F.; et al (December 2022, Biotechnology and Bioengineering)

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